70014625 female caucasian 29 atcc pcs  (ATCC)

Journal: iScience

Article Title: Human SKI component SKIV2L regulates telomeric DNA-RNA hybrids and prevents telomere fragility

doi: 10.1016/j.isci.2024.111096

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Article Snippet: Stable clone of adult female human cervical adenocarcinoma HeLa cell line with long telomeres (HeLa1.3 cells); adult male human fibrosarcoma HT1080 cell line overexpressing telomase (HT1080 super-telomerase cells, HT1080-ST) (kindly provided by T. de Lange and J. Lingner, respectively); adult female human osteosarcoma U2OS cell line (ATCC); human embryonic kidney HEK293FT cell line (ATCC); and human female fetal lung fibroblast IMR90 cell line (Coriel Institute) were cultured in DMEM medium supplemented with 10% (v/v) fetal bovine serum (FBS, Sigma-Aldrich, F2442) and maintained at 37°C in 5% (v/v) CO 2 .

Techniques: Virus, Recombinant, Protease Inhibitor, Reverse Transcription, Blocking Assay, Mass Spectrometry, SYBR Green Assay, Flow Cytometry, Imaging, Mutagenesis, Cell Cycle Assay, shRNA, Software

Journal: STAR Protocols

Article Title: An open access, machine learning pipeline for high-throughput quantification of cell morphology

doi: 10.1016/j.xpro.2022.101947

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Article Snippet: IMR-90 human female fetal lung fibroblasts , Coriell Institute for Medical Research , Cat # I90-10.

Techniques: Recombinant, Software, Microscopy, Imaging

Journal: bioRxiv

Article Title: Discovery of new senolytics using machine learning

doi: 10.1101/2022.04.26.489505

Figure Lengend Snippet: (A) Experimental setup of oncogene-induced senescence model with IMR90 ER RAS cells. Senescence was induced by addition of 4-OHT at 100 nM during thet duration of the experiment (eight days). Control and senescent cells were plated in a 96 well plate day five of 4-OHT induction. Top predicted compounds were added after multiwell seeding, and 72 hours afterwards, the cells were fixed, and the nuclei stained and counted. (B) Bar plot of OIS positive experimental control, ouabain, at 46.4 nM. Data is normalised to DMSO. Mean ± s.d. are shown from n=3 experiments. (C) Results from experimental validation of controls and the top 21 compounds from predicted to have senolytic action with P>44%. Three compounds out of the 21 displayed senolytic activity: ginkgetin, oleandrin, and periplocin. (D) Dose-response curves of the three newly found senolytic compounds. The senolytic index (SI) is defined as the ratio between the IC50 of control cells and the IC50 of senescent cells. Data is normalised to DMSO. Mean ± s.d. are shown from n=3 experiments. ( E ) Tanimoto distance from each of the three validated senolytics to each senolytic employed for training; distances were calculated using the RDKit descriptors that were employed in the training of machine learning models in and Supplementary Table 1.

Article Snippet: IMR90 female human foetal lung fibroblasts and A549 human lung adenocarcinoma cells were obtained from the American Type Culture Collection (ATCC, Manassas, VA).

Techniques: Control, Staining, Biomarker Discovery, Activity Assay